Keragaman Genetik Empat Populasi Arenga Pinnata Merr Berdasarkan Penanda Isozim = Genetic Diversity of Four Populations of Arenga pinnata Merr Revealed by Isozyme Markers

Aren tree or Sugar Palm (Arenga pinnata Merr) is one of bioethanol-producer which has advantages compared to other plant producing bioethanol. This study is aimed to determine the genetic diversity of aren tree populations in its natural distribution. Genetic diversity of four populations were investigated by isozyme markers with 4 enzyme systems namely Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Diaphorase (DIA) and 6-Phospogluconate dehydrogenase (6Pg). The number of alleles identified by 9 alleles are distributed on four polymorphic loci. The research revealed that mean alleles per locus was 2.2500. The mean effective number of alleles per polymorphic locus was 1.8377. All loci were of polymorphic. The mean genetic diversity within population was 0.4 381 and the proportion of genetic variation among populations was 0.0702. The UPGMA cluster analysis based on Nei's standard genetic distance reflected two main clusters. The first cluster consisted of Central Java and South Kalimantan populations; while second cluster included the populations of Bengkulu and North Sulawesi
= Aren (Arenga pinnata Merr) merupakan salah satu jenis penghasil bioetanol yang memiliki keunggulan dibanding dengan tumbuhan penghasil bioetanol lain. Penelitian ini bertujuan untuk mengetahui besamya keragaman genetik populasi-populasi aren dari sebaran alamnya. Keragaman genetik keempat populasi dideteksi dengan penanda genetik isozim dengan 4 sistem enzim yaitu Esterase (EST), Glutamate oxaloacetate transamirase (GOT), Diaphorase (DIA) dan 6-Phospo¬gluconate dehydrogenase (6Pg). Jumlah alel yang teridentifikasi sebanyak 9 alel yang tersebar pada empat lokus polimorfik. Rata-rata alel per lokus sebesar 2,2500. Rata-rata alel efektif per lokus sebesar 1,8377. Semua lokus bersifat polimorfik. Rata-rata keragaman genetik dalam populasi sebesar 0,4381 dan antar populasi sebesar 0,0702. Analisis klaster UPGMA berdasarkan jarak genetik standar Nei dapat membagi menjadi 2 klaster yaitu klaster pertama meliputi populasi Jawa Tengah dan Kalimantan Selatan; klaster kedua meliputi populasi Bengkulu dan Sulawesi Utara.

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Judul Seri

Jurnal Pemuliaan Tanaman Hutan

No. Panggil

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Penerbit

: ., 2011

Deskripsi Fisik

13-21 hal

Bahasa

English

ISBN/ISSN

16937147

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Info Detil Spesifik

Perpustakaan R.I. Ardi Koesoema

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