Development of Three Molecular Markers in Sengon (Falcataria moluccana (Miq.) Barneby & J.W.Grimes)

ABSTRACT

Sengon (Falcataria moluccana (Miq.) Barneby & J.W.Grimes) is one of the most important tree species of tropical forests in South-east Asia including Indonesia. Many studies have been conducted on the species, however most of the studies examined the breeding characteristics and its role in supplying soil nutrients. In order to facilitate genetics studies in sengon, this study was conducted. In our study, three molecular markers have succesfully developed. Five SCAR (Sequence Characterized Amplified Region) markers that provide simple and cost-effective method and 12 SNPs (Single Nucleotide Polymorphisms) in 3 sets of SNuPE (Single Nucleotide Primer Extension) analysis for higly reliable identification were constructed. The SCAR markers have low to moderate level of discrimination power, whereas 3 sets of SNuPE analysis have almost 100% (1,000 of DP) of discrimination power if all sets were used. Further development was ten microsatellite markers which have a high number of alleles (8 to 16) and 0.752 to 0.924 of expected heterozygosities. The five polymorphic SCAR markers and the twelve SNP markers in three sets of mulplexed SNuPE analysis will be useful for genotype identification in sengon, whereas the ten microsatellite markers will be a powerful tool for the study of molecular ecology, and population genetics, and conservation genetics of sengon.

Keywords: Sengon, Genetics studies, SCAR, Microsatellites, SNPs, SNuPE analysis

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Informasi Detil

Judul Seri

Prosiding INAFOR (International Conference of Indonesia Forestry Researchers) 2011

No. Panggil

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Penerbit

: sine nomine., 2012

Deskripsi Fisik

146-152

Bahasa

English

ISBN/ISSN

978-979-8452-45-1

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subyek

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