Seleksi bakteri penyakit nitrogen asal hutan Tanaman Akasia dan lahan bekas tambang batu bara pada bibit A. crassicarpa

The research was aimed at selecting rhizobia capable of fIXing optimum nitrogen biologically by means of symbiosis with Acacia crassicama seedlings in the nursery stage. Two biogeoclimatically-distinct areas were chosenfor nodules collection including Forest Subdistrict Gunung Kencana. Banten, Java and post coal mining area, Bukit Asam. Tanjung Enim, South Sumatra that represented plantation forest of acacias and marginal land, respectively. Observation showed that rhizobia had various performances, ranged from flat to domed, white-opaque to milky-to-watery-translucent, glistening or dull. Some colonies were even pink or reddish in colour and formed darker center of riblike marking. Colony size varied from 1 to 5 mm. Population also varied after 3 days of incubation in the dark at room temperature from 2,0x107 to 3,0x1010. for isolates S-80.I dan S-85.2, repectively, both isolates originatedfrom acacias plantation forest. Authentication study confirmed that all isolates were rhizobia shown by the presence of nodules in all seedlings inoculated with bacterial slurry. After 2.5 months, inoculation of 57 isolates showed various' growth respond on Acacia crassicama seedlings. Rhizobiulh was significantly involved in growth improvement, revealed by shoot dryweight (PO. 001), root dry weight (PO.OOI), shoot dryweight!rool dry weight ratio (PO.OOI), numbers of nodule (PO.OOI) and nodule pigmentation (P0.05). The average ofthe highest shoot dryweight was achieved by seedlings inoculated with S-70.2, that is 178 mg. Shoot dryweightlroot dry weight ratio ranged from 1.07-2.60. A lmo.\'t all seedlings inoculated with Rhizobium showed improved growth perfomance compared to that of controls with various potential in fixing nitrogen. Inoculation improved seedling growth up to 214. 51 percent compared to thai of controls. Five best-improved growths were seedlings inoculated with S-70.2, S-6I.3, 34.1,S-66.I dan S-63./, all were isolates of post coal mining land origins. More than onerhizobial strain could be isolatedfrom single host with various nitrogen fIXing potentials. The study revealed the importance of screening stage to obtain specific isolate compatible with A cacia cra.\'sicarpa that is efficient infixing nitrogen thus improvedplant growth.

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: .,

Deskripsi Fisik

Buletin Penelitian Hutan : No.636

Bahasa

Indonesia

ISBN/ISSN

1410-0649

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Perpustakaan R.I. Ardi Koesoema

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