Mating System of Calophyllum inophyllum Across Three DifferentForest Types
ABSTRACT
Tree improvement strategies have been initiated for Calophyllum inophyllum in order to produce high quality seeds that have high economic value for bio fuels. Genetic information is important to make the strategies more efficient. The aims of this study were to assess genetic diversity within and inter population and to study mating system of Calophyllum inophyllum across three different forest types using DNA markers. Leaf samples of both mature trees and seedlings were collected from Gunung Kidul (a plantation forest), Alas Purwo (a continuous natural forest) and Cilacap (a fragmented natural forest) and analyzed using 5 primers RAPD with 17 polymorphism loci. Results showed that level of gene diversity (h) of mature trees populations of Calophyllum inophyllum ranged between 0.132 (Alas Purwo) and 0.303 (Gunung Kidul). The h level increased at all offspring populations; it ranged between 0.303 (Cilacap) and 0.441 (Gunung Kidul). AMOVA analyses indicated that most variation (96.95%) was found within population and it was significantly associated with the value of genetic variation. An UPGMA dendrogram support the hypothesis that random mating has occurred at all the three forest types. Genetic diversity level and mating status across the different forests were briefly discussed.
Keywords: Genetic diversity, mating system, RAPD markers
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