Conversion of AFLP marker to sequence specific PCR marker in S. leprosula

ABSTRACT

Genetic marker can efficiently obtained by using amplified fragment length polymorphism (AFLP) fingerprinting. One AFLP fragment has been identified specific for S. leprosula. The AFLP marker was isolated from a gel, re-amplified, cloned and sequenced. Primer set was designed from these sequences and converted into a SCAR (Sequence Characterized Amplified Region) marker. The SCAR was used to screen samples of. S.leprosula from 14 widely separated populations in Indonesia. The marker was successfully applied to confirm the species S. leprosula.

Keywords: Genetic marker, AFLP, SCAR, S. leprosula

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Informasi Detil

Judul Seri

Prosiding INAFOR (International Conference of Indonesia Forestry Researchers) 2011

No. Panggil

//

Penerbit

: ., 2012

Deskripsi Fisik

100-107

Bahasa

English

ISBN/ISSN

978-979-8452-45-1

Klasifikasi

//

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subyek

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Pernyataan Tanggungjawab

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