Chloroplast DNA Distribution of Shorea leprosula, MIQ Revealed byMicrosatellite Markers

ABSTRACT

Shorea leprosula, Miq is a member of the Diptecarpaceae family. This species is ecologically and commercially important in the Indonesian. In the present study, we used chloroplast microsatellites (cpSSR) to study the distribution of chloroplast DNA haplotypes and to assess the genetic variation within and among populations of S. leprosula. Based on chloroplast microsatellite analysis the following haplotypes were detected for S. leprosula, namely haplotype H01 (ccmp3 =
101 bp and ccmp6 = 97 bp), H02 (ccmp3 = 100 bp and ccmp6 = 98 bp), and H03 (ccmp3 = 100 bp and ccmp6 = 97 bp). The haplotype variation of cpSSR was low in this study. The genetic differentiation (Gst) of S. leprosula was = 0.14, it was low in comparison to other studies on trees using maternally inherited markers. These values indicate the high efficiency of gene flow via seeds among populations of S. leprosula. Geographical distribution of haplotypes did not directly reflect the distribution of S. leprosula populations. Thus, haplotype distribution could not be used for genetic fingerprinting for detecting the geographical origin of S. leprosula.


Keywords: Dipterocarpaceae, shorea, chloroplast microsatellite, genetic variation

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Informasi Detil

Judul Seri

Prosiding INAFOR (International Conference of Indonesia Forestry Researchers) 2011

No. Panggil

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Penerbit

: ., 2012

Deskripsi Fisik

90-99

Bahasa

English

ISBN/ISSN

978-979-8452-45-1

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