PROPAGASI TIGAVARIETAS MURBEI MELALUI TEKNIK KULTUR JARINGAN(Propagation of Three Mulberry Varieties by means of Tissue Culture Technique)

Morus32Research in Propagation of three mulberry varieties through tissue culture technique aims to determine proper concentration of Benzyl amino purine (BAP) for the propagation of three mulberry ( sp.) varieties of KI 14, KI 29 and KI 41. Explants were obtained from auxillary buds of stem branches that were cultured using water medium. Prior to tissue culture experiment, explants were incubatedinMS0mediumwithoutgrowthregulator.Explantsfrom threemulberryvarieties(KI14,KI29, KI41)wereculturedinMurashigeandSkoog(MS)addedwithBAPconcentrationat2,2.5and3mg/lfor bud induction. Factorial in Completely Randomized Design in 5 replicates was used. The result showed that the fastest bud development was B V which obtained in combination treatment between BAP at 3 mg/landKI29variety.ThemostnumberousbudswereobtainedincombinationtreatmentatKI14variety and3mg/lBAP,whilethelongestshootswereobtainedinKI14variety.
Keywords:
BAP, tissue culture, mulberry, propagation, varieties

Penelitianpropagasitigavarietasmurbeimelaluikulturjaringanbertujuanuntukmengetahuikonsentrasi BenzylAmino Purin (BAP) yang tepat untuk perbanyakan murbei ( sp.) varietas KI 14, KI 29 dan KI 41. Eksplan diperoleh dari tunas aksilar dari cabang yang direndam air. Eksplan ditanam pada media MS0 (Media MS tanpa zat pengatur tumbuh). Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan pola faktorial. Faktor I adalah konsentrasi BAP (2, 2,5 dan 3 mg/l). Faktor II adalah varietas murbei (KI 14, KI 29 dan KI 41) dan setiap perlakuan diulang 5 kali. Hasil penelitian menunjukkanbahwaperlakuan yangtercepatbertunasadalahB V (BAP3mg/l,varietasKI29),jumlah tunasyangterbanyak diperolehpadaperlakuanvarietasKI14danperlakuanBAPkonsentrasi3mg/l,dan panjangtunasyangtertinggidiperolehpadavarietasKI14. Katakunci:Morus32BAP,kulturjaringan,murbei,propagation,varietas

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12

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1829-6327

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